Access to Oxygenated Monoterpenes via the Biotransformation of (R)-Limonene by Trichoderma harzianum and Saccharamyces cerevisiae

• Published in: Chemistry Africa Vol. 3; no. 3; pp. 647 - 656
• Main Authors: Ben Bnina, Enis, Daami-Remadi, Majda, Ben Jannet, Hichem
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.09.2020
• Subjects: Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Inorganic Chemistry; Organometallic Chemistry; Original Article; limonene; (; Bioconversion; Oxygenated monoterpenes
• ISSN: 2522-5758; 2522-5766
• DOI: 10.1007/s42250-020-00154-3

Microbial biotransformation is a pertinent strategy to overcome difficulties and problems arising from the chemical synthesis, in order to have access to regiospecific and stereospecific compounds and also explain the inactivity of essential oils or chemicals against some microorganisms. We evaluate in this research work, the bioconversion of ( R )-limonene dominating the chemical composition of peels essential oil of the Tunisian Citrus aurantium by Trichoderma harzianum and Saccharomyces cerevisiae . In order to understand the exact and specific effects of these two yeasts on ( R )-limonene, a negative control in the presence of air oxygen was carried out. Six oxygenated monoterpenes were produced and identified by GC–MS. Following the same protocol in the presence of these two yeasts, eight and seven secondary metabolites were formed, respectively. The results showed specific bioconversions and the fungi used involved to complete the full conversion of limonene and catalyze certain successive reactions in a specific way.