An innovative intramedullary bone graft harvesting concept as a fundamental component of scaffold-guided bone regeneration: A preclinical in vivo validation

• Published in: Journal of orthopaedic translation Vol. 47; pp. 1 - 14
• Main Authors: Laubach, Markus, Herath, Buddhi, Suresh, Sinduja, Saifzadeh, Siamak, Dargaville, Bronwin L., Cometta, Silvia, Schemenz, Victoria, Wille, Marie-Luise, McGovern, Jacqui, Hutmacher, Dietmar W., Medeiros Savi, Flavia, Bock, Nathalie
• Format: Journal Article
• Language: English
• Published: Singapore Elsevier B.V 01.07.2024; Chinese Speaking Orthopaedic Society
• Subjects: Bone graft; Bone regeneration; Intramedullary harvesting; Original; Polycaprolactone; Scaffold; Voronoi; Scaffold; Intramedullary harvesting; Bone graft; Bone regeneration; Polycaprolactone; Voronoi
• ISSN: 2214-031X; 2214-0328
• DOI: 10.1016/j.jot.2024.05.002

The deployment of bone grafts (BGs) is critical to the success of scaffold-guided bone regeneration (SGBR) of large bone defects. It is thus critical to provide harvesting devices that maximize osteogenic capacity of the autograft while also minimizing graft damage during collection. As an alternative to the Reamer-Irrigator-Aspirator 2 (RIA 2) system – the gold standard for large-volume graft harvesting used in orthopaedic clinics today – a novel intramedullary BG harvesting concept has been preclinically introduced and referred to as the ARA (aspirator + reaming-aspiration) concept. The ARA concept uses aspiration of the intramedullary content, followed by medullary reaming-aspiration of the endosteal bone. This concept allows greater customization of BG harvesting conditions vis-à-vis the RIA 2 system. Following its successful in vitro validation, we hypothesized that an ARA concept-collected BG would have comparable in vivo osteogenic capacity compared to the RIA 2 system-collected BG. We used 3D-printed, medical-grade polycaprolactone-hydroxyapatite (mPCL-HA, wt 96 %:4 %) scaffolds with a Voronoi design, loaded with or without different sheep-harvested BGs and tested them in an ectopic bone formation rat model for up to 8 weeks. Active bone regeneration was observed throughout the scaffold-BG constructs, particularly on the surface of the bone chips with endochondral bone formation, and highly vascularized tissue formed within the fully interconnected pore architecture. There were no differences between the BGs derived from the RIA 2 system and the ARA concept in new bone volume formation and in compression tests (Young's modulus, p = 0.74; yield strength, p = 0.50). These results highlight that the osteogenic capacities of the mPCL-HA Voronoi scaffold loaded with BGs from the ARA concept and the RIA 2 system are equivalent. In conclusion, the ARA concept offers a promising alternative to the RIA 2 system for harvesting BGs to be clinically integrated into SGBR strategies. Our results show that biodegradable composite scaffolds loaded with BGs from the novel intramedullary harvesting concept and the RIA 2 system have equivalent osteogenic capacity. Thus, the innovative, highly intuitive intramedullary harvesting concept offers a promising alternative to the RIA 2 system for harvesting bone grafts, which are an important component for the routine translation of SGBR concepts into clinical practice. [Display omitted]