Novel cyclic undecapeptides immobilized on reduced graphene oxide surface for enhanced antibacterial properties

• Published in: International journal of environmental science and technology (Tehran) Vol. 21; no. 2; pp. 1481 - 1492
• Main Authors: Farrag, H. N., Zheng, A. L. T., Sabidi, S., Wu, Y., Ikeno, S., Maeda, T., Andou, Y., Kato, T.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 2024
• Subjects: Aquatic Pollution; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Science and Engineering; Original Paper; Soil Science & Conservation; Waste Water Technology; Water Management; Water Pollution Control; Cyclic undecapeptides; Synergistic; Antibacterial; Reduced graphene oxide
• ISSN: 1735-1472; 1735-2630
• DOI: 10.1007/s13762-023-05035-z

The incorporation of antimicrobial peptides (AMPs) in graphene-based materials is a growing strategy to overcome the shortcomings presented by conventional antimicrobial nanomaterials derived from metal/metal oxide. In this study, novel cyclic undecapeptides CH 1 and CW 2 (KRQRFYFRQRK and QNKRFYFRKNQ, respectively) were successfully synthesized. The cyclic peptides were then immobilized on the reduced graphene oxide (rGO) surface using a water-soluble carbodiimide (EDCI/HCl) linker. The structural, morphology, and chemical characterizations of the antimicrobial graphene material were carried out using SEM, Raman, FTIR, XRD, and XPS. From our result, the peptide/rGO composite showed an improved antibacterial effect towards gram-positive ( Staphylococcus aureus ) and gram-negative ( Pseudomonas aeruginosa ). The enhanced antibacterial property is attributed to the synergistic effect of the sharp edges of rGO and the amphipathic moieties of the cyclic peptides.