Immobilization of GOD on Ppy-PVS Composite Film for Determination of Glucose: A Comparative Study of Phosphate and Acetate Buffers

• Published in: International journal of polymeric materials Vol. 56; no. 11; pp. 1051 - 1065
• Main Authors: Gade, V. K., Shirale, D. J., Gaikwad, P. D., Savale, P. A., Kakde, K. P., Kharat, H. J., Shirsat, M. D.
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 14.08.2007; Taylor & Francis
• Subjects: Applied sciences; Biological and medical sciences; biosensor; Biosensors; Biotechnology; composite film; crosslinking; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; glucose-oxidase; immobilization; Immobilization of enzymes and other molecules; Immobilization techniques; Methods. Procedures. Technologies; Organic polymers; Physicochemistry of polymers; Properties and characterization; Special properties (catalyst, reagent or carrier); Various methods and equipments; Composite film; Glucose oxidase; Electrical conductivity; immobilization; Electrical properties; Support; Enzyme; Crosslinking; glucose-oxidase; Glucose; Experimental study; Electrochemical polymerization; Conducting polymers; Polyelectrolyte; Sulfonate polymer; Sensitivity; Medium effect; Biosensor; Morphology; Preparation; Oxidoreductases; Immobilized enzyme; Pyrrole polymer
• ISSN: 0091-4037; 1563-535X
• DOI: 10.1080/01904160701219971

The Polypyrrole-polyvinylsulphonate-glucose oxidase (Ppy-PVS-GOD) biosensor for determination of glucose has been described in the present investigation. The enzyme, glucose oxidase (GOD) was immobilized by crosslinking via glutaraldehyde on a polypyrrole-polyvinyl sulphonate (Ppy-PVS) composite film. The Ppy-PVS film was electrochemically synthesized on indium-tin-oxide (ITO)-coated glass plate. The synthesized composite films were characterized using galvanostatic electrochemical technique, electrical conductivity, UV-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The crosslinking of enzyme and porous morphology of the polymer film leads to high enzyme loading and an increase in lifetime, stability, and fast response time of the enzyme electrode. Characterization of resulting amperometric biosensor for the estimation of glucose has been experimentally determined in terms of linear response range, optimum pH, applied potential, and shelf-life. These Ppy-PVS-GOD electrodes can be used for glucose estimation from 1 to 50 mM and have a shelf-life of about 5-6 weeks at 4°C. The sensitivity of Ppy-PVS-GOD electrode in phosphate and acetate buffer has been studied. It was found that the phosphate buffer gives fast response as compared to acetate buffer in amperometric measurements.