High-performance liquid chromatography with fluorescence detection for the simultaneous determination of 3,4-methylenedioxymethamphetamine, methamphetamine and their metabolites in human hair using DIB-Cl as a label
This paper describes a highly sensitive HPLC method for the simultaneous determination of 3,4‐methylenedioxymethamphetamine (MDMA), 3,4‐methylenedioxyamphetamine (MDA), amphetamine (AP) and methamphetamine (MP) in human hair samples. The amphetamines investigated were derivatized with the fluorescent...
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Published in | Biomedical chromatography Vol. 18; no. 3; pp. 202 - 204 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
01.04.2004
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Subjects | |
Online Access | Get full text |
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Summary: | This paper describes a highly sensitive HPLC method for the simultaneous determination of 3,4‐methylenedioxymethamphetamine (MDMA), 3,4‐methylenedioxyamphetamine (MDA), amphetamine (AP) and methamphetamine (MP) in human hair samples. The amphetamines investigated were derivatized with the fluorescent reagent, DIB‐Cl to yield highly fluorescent DIB‐derivatives, which were then analyzed by HPLC with fluorescence detection at excitation and emission wavelengths of 325 nm and 430 nm, respectively. The separation was achieved on an ODS column with an isocratic mobile phase composed of acetonitrile‐methanol‐water (30:40:30, v/v/v). The limits of detection for the four compounds obtained by the proposed method ranged from 11 to 200 pg/mg. The method was successfully applied to the determination of MDMA and MDA in hair samples obtained from MDMA abuser. Copyright © 2004 John Wiley & Sons, Ltd. |
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Bibliography: | istex:8A9CB35E830A014CA0FE42115685B603A45AA6E6 ArticleID:BMC374 ark:/67375/WNG-X59WT47N-K ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0269-3879 1099-0801 |
DOI: | 10.1002/bmc.374 |