Stimulation of cellular ingestion by basic proteins in vitro

The ingestion of carbon and benzpyrene particles in vitro by rat peritoneal macrophages, baby hamster kidney fibroblasts (BHK-21) and mouse L-cells has been shown to be significantly stimulated by the inclusion of histone or polylysine in the culture medium. Parallel studies using methylated bovine...

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Bibliographic Details
Published inBritish journal of cancer Vol. 33; no. 4; pp. 392 - 399
Main Authors Westwood, F R, Longstaff, E
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 01.04.1976
Subjects
Animals
Benzopyrenes
Carbon
Cells, Cultured
Female
Fibroblasts - drug effects
Fibroblasts - physiology
Fibroblasts - ultrastructure
Histones - pharmacology
L Cells (Cell Line)
Macrophages - drug effects
Macrophages - physiology
Male
Neoplasms - chemically induced
Peptides - pharmacology
Phagocytosis - drug effects
Polylysine - pharmacology
Rats
Serum Albumin, Bovine - pharmacology
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Summary:The ingestion of carbon and benzpyrene particles in vitro by rat peritoneal macrophages, baby hamster kidney fibroblasts (BHK-21) and mouse L-cells has been shown to be significantly stimulated by the inclusion of histone or polylysine in the culture medium. Parallel studies using methylated bovine albumin did not significantly stimulate carbon or benzpyrene uptake relative to untreated control cultures. Incubation of carbon particles with histone before inclusion in the culture medium of macrophages resulted in the same degree of uptake as in the cultures where carbon and histone were added independently of each other. The implications of these findings to in vivo chemical carcinogenesis are examined.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1976.64