Molecular intrinsic proximal interaction infer oxidative stress and apoptosis modulated in vivo biocompatibility of P.niruri contrived antibacterial iron oxide nanoparticles with zebrafish

• Published in: Environmental pollution (1987) Vol. 267; p. 115482
• Main Authors: Sheel, Rishav, Kumari, Puja, Panda, Pritam Kumar, Jawed Ansari, Md Danish, Patel, Paritosh, Singh, Sonal, Kumari, Baby, Sarkar, Biplab, Mallick, M. Anwar, Verma, Suresh K.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2020
• Subjects: Apoptosis; Danio rerio; Green synthesis; Iron oxide nanoparticles; Nanotoxicity; Oxidative stress; Nanotoxicity; Oxidative stress; Green synthesis; Iron oxide nanoparticles; Danio rerio; Apoptosis
• ISSN: 0269-7491; 1873-6424; 1873-6424
• DOI: 10.1016/j.envpol.2020.115482

Extensive use of magnetic iron oxide (magnetite) nanoparticles (IONP) has raised concerns about their biocompatibility. It has also stimulated the search for its green synthesis with greater biocompatibility. Addressing the issue, this study investigates the molecular nanotoxicity of IONP with embryonic and adult zebrafish, and reveal novel green fabrication of iron oxide nanoparticles (P-IONP) using medicinal plant extract of Phyllanthus niruri. The synthesized P-IONP was having a size of 42 ± 08 nm and a zeta potential of −38 ± 06 mV with hydrodynamic diameter of 109 ± 09 nm and 90emu/g magnetic saturation value. High antibacterial efficacy of P-IONP was found against E.coli. Comparative in vivo biocompatibility assessment with zebrafish confirmed higher biocompatibility of P-IONP compared to commercial C-IONP in the relevance of mortality rate, hatching rate, heart rate, and morphological abnormalities. LC50 of P-IONP and C-IONP was 202 μg/ml and 126 μg/ml, respectively. Molecular nano-biocompatibility analysis revealed the phenomenon as an effect of induced apoptosis lead by dysregulation of induced oxidative stress due to structural and functional influence of IONP to Sod1 and Tp53 proteins through intrinsic atomic interaction. [Display omitted] •Phytochemicals of P. niruri synthesized 42 ± 08 nm P-IONP nanoparticles.•P-IONP were more biocompatible than commercial C-IONP assessed in Zebrafish modal.•IONP-Sod1 & IONP-Tp53 proximal interaction alter oxidative stress and apoptosis.•Ser, his, lys and pro interact via H-bond and hydrophobic interaction. Green synthesized iron oxide nanoparticles from P.niruri exhibited antibacterial effect with higher biocompatibility with zebrafish. The biocompatibility is modulated by induced oxidative stress lead apoptosis triggerd by proximal intrinsic atomic interaction with Sod 1 and Tp53 proteins mediated through amino acid like Serine, histidine, lysin and proline via H-bond and hydrophobic interaction.