Properties and localization of endothelin-1-specific receptors in rat testicles

The characterization and localization of rat testicular endothelin receptors were studied. Receptor binding assay with radiolabeled members of the endothelin family revealed that endothelin-1-specific receptors were present in rat testes; a maximal binding capacity of 250 +/- 62 fmol/mg protein and...

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Bibliographic Details
Published inThe American journal of physiology Vol. 263; no. 1 Pt 2; p. R15
Main Authors Sakaguchi, H, Kozuka, M, Hirose, S, Ito, T, Hagiwara, H
Format Journal Article
LanguageEnglish
Published United States 01.07.1992
Subjects
Animals
Binding, Competitive
Endothelins - metabolism
Ligands
Male
Rats
Rats, Inbred Strains
Receptors, Cell Surface - metabolism
Receptors, Endothelin
Testis - metabolism
Tissue Distribution
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Summary:The characterization and localization of rat testicular endothelin receptors were studied. Receptor binding assay with radiolabeled members of the endothelin family revealed that endothelin-1-specific receptors were present in rat testes; a maximal binding capacity of 250 +/- 62 fmol/mg protein and a dissociation constant value of 0.35 +/- 0.06 nM were calculated from the Scatchard plot. The affinities for endothelin analogues were endothelin-1 = endothelin-2 much greater than endothelin-3 much greater big endothelin-1 for the membrane-bound receptors. Receptors of endothelin-1 were localized in peritubular myoid cells and interstitial cells of rat testis by 125I-labeled endothelin-1 autoradiography; the receptors were undetectable in spermatogenic cells. The presence of endothelin-1 receptors in the rat testis raises the possibility that one or more of the endothelins may play a physiological role in the regulation of testicular function.
ISSN:0002-9513
DOI:10.1152/ajpregu.1992.263.1.R15