Fine mapping of bovine herpesvirus 1 (BHV-1) glycoprotein C neutralizing epitopes by type-specific monoclonal antibodies and synthetic peptides

Bovine herpesvirus glycoprotein C (gC) functions as a major virus attachment protein. Here, two BHV-1 gC-specific epitopes that are specified by complement-dependent neutralizing MAbs are mapped. The BHV-1 gC-specific peptides and MAbs were used to specifically localize continuous epitopes by direct...

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Bibliographic Details
Published inVeterinary microbiology Vol. 58; no. 2; pp. 309 - 314
Main Author Chowdhury, S.I.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.11.1997
Subjects
Animals
Antibodies, Monoclonal
Antibody Specificity
Antigens, Viral
Bovine herpes virus
Cattle
Enzyme-Linked Immunosorbent Assay
Epitopes
Herpesvirus 1, Bovine - isolation & purification
Neutralization Tests
Peptide Fragments - chemical synthesis
Viral Proteins - analysis
Viral Proteins - chemistry
Viral Proteins - immunology
Epitopes
Bovine herpes virus
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Summary:Bovine herpesvirus glycoprotein C (gC) functions as a major virus attachment protein. Here, two BHV-1 gC-specific epitopes that are specified by complement-dependent neutralizing MAbs are mapped. The BHV-1 gC-specific peptides and MAbs were used to specifically localize continuous epitopes by direct binding to the MAbs and by blocking the Mab reactivity (competitive ELISA) to authentic viral antigen. The results of competitive ELISA indicated that the complement-dependent neutralizing epitopes recognized by MAbs F2 and 24 were located between BHV-1 gC amino acids (aa) 47–69 and (aa) 109–119, respectively.
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ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(97)00146-6