Studies on the Oxidative Phosphorylation in Higher Plants. Part 2

• Published in: Nippon Nōgeikagaku Kaishi Vol. 27; no. 7; pp. 429 - 433
• Main Authors: MARUO, Bunji, OMURA, Takao, MATSUHASHI, Michio, FUKUSHI, Sadao
• Format: Journal Article
• Language: Japanese
• Published: Japan Society for Bioscience, Biotechnology, and Agrochemistry 1953
• ISSN: 0002-1407; 1883-6844
• DOI: 10.1271/nogeikagaku1924.27.429

Decrease of inorganic ortho phosphate in aerobic condition by the whole homogenate of Phaseolus Mungo was reported previously(1). The products of the oxidative phosphorylation in the same system were investigated using P32 as a tracer. Fractionation of the reaction products were carried out as in Fig. 1, and the amounts of inorganic phosphate, tolal phosphate 7 min. phosphate and the relative radio activities of each fraction were listed in Table 1, and P32/P ratioes were shown in Table 2. Figs. 2_??_5 are paper chromatograms and their auto radiograms of these fractions. These results indicatethat phosphate radicals esterifed by this system are transferred mainly in the Ba-soluble and the Ba-insoluble phosphate compoumds. From these results the authors conclude that the oxidative phosphorylation is really accomplished by this system.