Arthrobacter crusticola sp. nov., Isolated from Biological Soil Crusts in the Mu Us Sandy Land, China

• Published in: Current microbiology Vol. 77; no. 9; pp. 2042 - 2048
• Main Authors: Liu, Lei, Liang, Lixiong, He, Shanwen, Xu, Lijun, Chi, Miao, Shi, Shaowen, Zhang, XiaoXia, Li, Lubin
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.09.2020; Springer Nature B.V
• Subjects: Alanine; Arthrobacter; Arthrobacter - genetics; Bacterial Typing Techniques; Biodiversity; Biomedical and Life Sciences; Biotechnology; Cardiolipin; China; Crusts; Deoxyribonucleic acid; Desertification; DNA; DNA, Bacterial - genetics; Fatty Acids; Galactose; Genomes; Glutamic acid; Hybridization; Life Sciences; Lipids; Lysine; Menaquinones; Microbiology; New species; Nucleic Acid Hybridization; Nucleotides; Peptidoglycans; Phosphatidylglycerol; Phosphatidylinositol; Phospholipids; Phylogeny; Ribose; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sand; Sandy soils; Sequence Analysis, DNA; Soil; Soils; Sugar; China
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-020-02070-8

Mu Us Sandy Land in China is a very fragile ecological environment due to serious desertification. While attempting to gain insights into the biodiversity of biological soil crusts of Mu Us Sandy Land, a novel bacterial strain, SLN-3 T , was isolated. It was phylogenetically placed into the genus Arthrobacter within the family Micrococcaceae based on its 16S rRNA gene sequence. The most closely related species were Arthrobacter ruber MDB1-42 T (98.6%) and Arthrobacter agilis DSM 20550 T (98.3%). Cells of the novel species were Gram-stain-positive, aerobic, and non-endospore-forming. The values of average nucleotide identity and the digital DNA-DNA hybridization between SLN-3 T and MDB1-42 T were 84.9% and 21.3%, respectively. The draft genome size of strain SLN-3 T was 3.67 Mb, and its genomic G+C content was 68.1%. The predominant cellular fatty acids were anteiso-C 15:0 and C 17:0 anteiso. Glucose, galactose, and ribose were the whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipid, and phospholipid. The peptidoglycan contained lysine, glutamic acid, and alanine. The predominant menaquinone was MK-9(H 2 ). Based on the data from the chemotaxonomic, phylogenetic, and phenotypic evidence, a novel species named Arthrobacter crusticola sp. nov is proposed, whose type strain is SLN-3 T (= ACCC 61595 T  = JCM 33723 T ).