Age‐stage, two‐sex life table and transcriptome analysis reveal the adaptation of Liriomyza trifolii to different host plants

• Published in: Entomologia experimentalis et applicata Vol. 173; no. 1; pp. 86 - 94
• Main Authors: Yan, Yu‐Qing, Chang, Ya‐Wen, Gong, Wei‐Rong, Hu, Jie, Du, Yu‐Zhou
• Format: Journal Article
• Language: English
• Published: Amsterdam Wiley Subscription Services, Inc 01.01.2025
• Subjects: Adaptation; age‐stage; Agricultural practices; Beans; Biopesticides; Crop rotation; cytochrome P-450; Cytochrome P450; Cytochromes P450; differentially expressed genes; Down-regulation; Fecundity; Flies; gene expression regulation; Genes; Glutathione; glutathione transferase; Gourds; host plant; Host plants; Insects; Integrated pest management; Invasiveness; Life tables; Liriomyza trifolii; longevity; Luffa aegyptiaca; mortality; Pest control; Pesticides; Sex; Tables (data); transcriptome; Transcriptomes; transcriptomics; Trypsin; two‐sex life table
• ISSN: 0013-8703; 1570-7458
• DOI: 10.1111/eea.13515

Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) is a polyphagous insect that is widely known for its invasiveness. Understanding the adaptation of L. trifolii to different host plants is critical in formulating effective approaches for integrated pest management (IPM). In this study, the effects of various host plants on L. trifolii were investigated by age‐stage, two‐sex life tables and transcriptome analysis. Our results show that the growth rate of immature L. trifolii on sponge gourd increased significantly relative to bean, but mortality was high. Mature L. trifolii adapted to sponge gourd had significantly increased longevity as compared to flies adapted to bean but exhibited reduced fecundity. The net reproductive rate, the intrinsic rate of increase, and the finite rate of increase of L. trifolii adapted to sponge gourd were significantly lower than those of flies adapted to bean. Transcriptome analysis showed the presence of 150 up‐ and 617 downregulated differentially expressed genes in L. trifolii adapted to sponge gourd as compared to flies adapted to bean. Genes encoding glutathione‐S‐transferase, cytochrome P450, and trypsin were significantly downregulated in L. trifolii adapted to sponge gourd as compared to bean. This study provides valuable insight into host plant effects on L. trifolii and provides a basis for the subsequent development of IPM measures such as push and pull, crop rotation, and biopesticide development. Life tables and transcriptome analysis were used to explore the adaptive ability of Liriomyza trifolii (Diptera: Agromyzidae) to various hosts. The net reproductive rate, intrinsic rate of increase, and finite rate of increase of L. trifolii on sponge gourd were lower than on bean. Glutathione‐S‐transferase, cytochrome P450 and trypsin related genes were differentially expressed between the two populations. The results will help understand future occurrence in the field and develop control measures.