Identification and expression analysis of caffeoyl-coenzyme A O-methyltransferase family genes related to lignin biosynthesis in tea plant (Camellia sinensis)
Tea plant, an economically important crop, is used in producing tea, which is a non-alcoholic beverage. Lignin, the second most abundant component of the cell wall, reduces the tenderness of tea leaves and affects tea quality. Caffeoyl-coenzyme A O -methyltransferase (CCoAOMT) involved in lignin bio...
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Published in | Protoplasma Vol. 258; no. 1; pp. 115 - 127 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Vienna
Springer Vienna
2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Tea plant, an economically important crop, is used in producing tea, which is a non-alcoholic beverage. Lignin, the second most abundant component of the cell wall, reduces the tenderness of tea leaves and affects tea quality. Caffeoyl-coenzyme A
O
-methyltransferase (CCoAOMT) involved in lignin biosynthesis affects the efficiency of lignin synthesis and lignin composition. A total of 10
CsCCoAOMTs
were identified based on tea plant genome. Systematic analysis of
CCoAOMTs
was conducted for its physicochemical properties, phylogenetic relationships, conserved motifs, gene structure, and promoter
cis
-element prediction. Phylogenetic analysis suggested that all the CsCCoAOMT proteins can be categorized into three clades. The promoters of six
CsCCoAOMT
genes possessed lignin-specific
cis
-elements, indicating they are possibly essential for lignin biosynthesis. According to the distinct tempo-spatial expression profiles, five genes were substantially expressed in eight tested tissues. Most
CsCCoAOMT
genes were expressed in stems and leaves in three tea plant cultivars ‘Longjing 43,’ ‘Anjibaicha,’ and ‘Fudingdabai’ by RT-qPCR detection and analysis. The expression levels of two genes (
CsCCoAOMT5
and
CsCCoAOMT6
) were higher than those of the other genes. The expression levels of most
CsCCoAOMT
genes in ‘Longjing 43’ were significantly higher than that those in ‘Anjibaicha’ and ‘Fudingdabai.’ Correlation analysis revealed that only the expression levels of
CsCCoAOMT6
were positively correlated with lignin content in the leaves and stems. These results lay a foundation for the future exploration of the roles of
CsCCoAOMTs
in lignin biosynthesis in tea plant. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0033-183X 1615-6102 |
DOI: | 10.1007/s00709-020-01555-4 |