Characterization of mouse model-derived osteosarcoma (OS) cells in vitro and in vivo

Osteosarcoma (OS) is the most common primary tumor of bone with a high incidence in children. Treatment options for OS are limited, and once metastasized, the prognosis is very poor. Genetically engineered mouse models (GEMMs) are valuable tools to understand the mechanisms of tumorigenesis and to t...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1267; p. 297
Main Authors Uluçkan, Özge, Bakiri, Latifa, Wagner, Erwin F
Format Journal Article
LanguageEnglish
Published United States 2015
Subjects
Alkaline Phosphatase - metabolism
Animals
Anthraquinones - metabolism
Bone Neoplasms - pathology
Carcinogenesis
Cell Line, Tumor
Cell Separation
Cell Transformation, Neoplastic
Disease Models, Animal
Mice
Osteosarcoma - pathology
Staining and Labeling
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Summary:Osteosarcoma (OS) is the most common primary tumor of bone with a high incidence in children. Treatment options for OS are limited, and once metastasized, the prognosis is very poor. Genetically engineered mouse models (GEMMs) are valuable tools to understand the mechanisms of tumorigenesis and to test possible therapies. In this chapter, we summarize the methods related to the isolation, characterization, and transplantation of OS cells obtained from GEMMs.
ISSN:1940-6029
DOI:10.1007/978-1-4939-2297-0_14