Characterization of 16S rRNA methylase genes in Enterobacterales and Pseudomonas aeruginosa in Athens Metropolitan area, 2015–2016
The aim of this study was to characterize the 16S rRNA methylase (RMT) genes in aminoglycoside-resistant Enterobacterales and Pseudomonas aeruginosa isolates in 2015–2016 in hospitals in Athens, Greece. Single-patient, Gram-negative clinical isolates resistant to both amikacin and gentamicin ( n = 2...
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Published in | European journal of clinical microbiology & infectious diseases Vol. 40; no. 1; pp. 111 - 121 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The aim of this study was to characterize the 16S rRNA methylase (RMT) genes in aminoglycoside-resistant Enterobacterales and
Pseudomonas aeruginosa
isolates in 2015–2016 in hospitals in Athens, Greece. Single-patient, Gram-negative clinical isolates resistant to both amikacin and gentamicin (
n
= 292) were consecutively collected during a two-year period (2015–2016) in five tertiary care hospitals in Athens. RMT genes were detected by PCR. In all RMT-producing isolates, ESBL and carbapenemase production was confirmed by PCR, and the clonal relatedness and the plasmid contents were also characterized. None of the 138
P. aeruginosa
isolates harbored any of the RMT genes surveyed although some were highly resistant to aminoglycosides (MICs > = 512 mg/L). Among 154 Enterobacterales, 31
Providencia stuartii
(93.9%), 42
Klebsiella pneumoniae
(37.8%), six
Proteus mirabilis
(75%), and two
Escherichia coli
(100%) isolates were confirmed as highly resistant to amikacin, gentamicin, and tobramycin with MICs ≥ 512 mg/L, harboring mainly the
rmtB
(98.8%). All were carbapenemase producers.
P. stuartii
,
P. mirabilis
, and
E. coli
produced VIM-type carbapenemases.
K. pneumoniae
produced KPC- (
n
= 34, 81.0%), OXA-48 (
n
= 4, 9.5%), KPC- and VIM- (
n
= 3, 7.1%), or only VIM-type (
n
= 1, 2.4%) enzymes. Two groups of similar IncC plasmids were detected one harboring
rmtB1
,
bla
VEB-1
,
bla
OXA-10
, and
bla
TEM-1
, and the other additionally
bla
VIM-1
and
bla
SHV-5
. Among RMT-producing Enterobacterales,
rmtB1
predominated and was associated with carbapenemase-encoding gene(s). Similar IncC plasmids carrying a multiresistant region, including ESBL genes, and in the case of VIM-producing isolates, the
bla
VIM-1
, were responsible for this dissemination. The co-dissemination of these genes poses a public health threat. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0934-9723 1435-4373 |
DOI: | 10.1007/s10096-020-04006-3 |