Diarrheagenic Escherichia coli isolates from neonatal alpacas mainly display F17 fimbriae adhesion gene

• Published in: Tropical animal health and production Vol. 52; no. 6; pp. 3917 - 3921
• Main Authors: Siuce, Juan, Maturrano, Lenin, Wheeler, Jane C., Rosadio, Raul
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.11.2020; Springer Nature B.V
• Subjects: Alpacas; Animals; Animals, Domestic; Biomedical and Life Sciences; Camelidae; Camelids, New World; Dehydration; Diarrhea - epidemiology; Diarrhea - microbiology; Diarrhea - veterinary; E coli; Escherichia coli; Escherichia coli - isolation & purification; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Escherichia coli Proteins - analysis; Fimbriae, Bacterial - genetics; Genes; Lethargy; Life Sciences; Neonates; Pili; Rectum; Septicemia; Short Communications; Veterinary Medicine/Veterinary Science; Virulence; Zoology; Alpacas; EPEC; Fimbriae genes; EHEC strains
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-020-02415-2

Neonatal domestic South American Camelid llamas and alpacas suffer from an enteric disease complex characterized by abdominal distention, lethargy, dehydration, and eventual fatal septicemia. Analysis of rectal swabs from neonatal alpacas suffering clinical diarrheas has constantly isolated Escherichia coli , mainly the EPEC and EHEC pathotypes. The present communication reports the results of molecular analysis of 226 E. coli strains from neonatal alpaca rectal swabs. The isolates were initially tested by multiple PCR, to identify E. coli virulence genes eae , bfp , Lt , Stx1 , Stx2 , sta , stab , and lt genes and a similar test to detect F4, F5, F6, F17, and F41fimbriae adhesin genes. Forty-two of the 226 (18.5%) isolates tested positive for at least one pathogenic gene, 25 of the 42 were classified as EPEC (3 positives for only eae and 22 for both eae and bfp ) and the remaining 17 were classified as EHEC. Twenty-four (57%) of the 42 isolates tested positive to F17 adhesin while one was positive for both F6 and F17.