Homogeneous time-resolved fluoroimmunoassay of thyroxin in serum

We describe a rapid, simple nonseparation fluoroimmunoassay for determination of thyroxin in serum. The assay is based on the labeling of thyroxin directly with a fluorescent europium chelate, the fluorescence of which is quenched on binding to an antithyroxin antibody. With the assay buffer we used...

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Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 34; no. 11; pp. 2320 - 2322
Main Authors Hemmila, I, Malminen, O, Mikola, H, Lovgren, T
Format Journal Article
LanguageEnglish
Published Washington, DC Am Assoc Clin Chem 01.11.1988
American Association for Clinical Chemistry
Subjects
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Antibodies, Monoclonal
Biological and medical sciences
Europium
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Humans
Proteins
Radioimmunoassay
Thyroxine - blood
Time Factors
Assay
Biological fluid
Time resolution
Coupled method
Radioimmunoassay
Aminoacid derivative hormone
Thyroxine
Serum
Comparative study
Blood
Fluorescence spectrometry
Immunological method
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Summary:We describe a rapid, simple nonseparation fluoroimmunoassay for determination of thyroxin in serum. The assay is based on the labeling of thyroxin directly with a fluorescent europium chelate, the fluorescence of which is quenched on binding to an antithyroxin antibody. With the assay buffer we used, maximum quenching is 90%. The rapid achievement of equilibrium in the assay solution, regardless of the sequence of reagent additions, allows fast measurement of thyroxin. Precision was good (CV less than 5%) within the clinical range for total thyroxin (50-300 nmol/L), and results correlated well with those by a commercial radioimmunoassay.
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/34.11.2320