Expression of UMP1 is inducible by DNA damage and required for resistance of S. cerevisiae cells to UV light

It has recently been shown that the UMP1 gene of Saccharomyces cerevisiae encodes a small. short-lived protein engaged in 20S proteasome formation. The results presented in this paper demonstrate that ULMP1 expression is induced by the DNA damaging agents methyl methanesulfonate (MMS) and UV light a...

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Published inCurrent genetics Vol. 38; no. 2; pp. 53 - 59
Main Authors Mieczkowski, P, Dajewski, W, Podlaska, A, Skoneczna, A, Ciesla, Z, Sledziewska-Gójska, E
Format Journal Article
LanguageEnglish
Published United States 01.08.2000
Subjects
Cysteine Endopeptidases - metabolism
DNA Damage
Gene Expression Regulation, Fungal
Hydroxyurea - pharmacology
Methyl Methanesulfonate - pharmacology
Molecular Chaperones - biosynthesis
Molecular Chaperones - genetics
Multienzyme Complexes - metabolism
Mutagens
Proteasome Endopeptidase Complex
Radiation Tolerance - genetics
Saccharomyces cerevisiae - radiation effects
Ultraviolet Rays
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Summary:It has recently been shown that the UMP1 gene of Saccharomyces cerevisiae encodes a small. short-lived protein engaged in 20S proteasome formation. The results presented in this paper demonstrate that ULMP1 expression is induced by the DNA damaging agents methyl methanesulfonate (MMS) and UV light as well as by hydroxyurea (HU), an inhibitor of DNA replication. MMS induction of UMP1 expression occurs at the transcriptional level and is independent of the activity of the regulatory checkpoint kinases encoded by MEC1. RAD53 or DUN1. It is also shown that the disruption of UMP1 causes increased sensitivity of yeast cells to killing by UV radiation, but only slight sensitivity to HU treatment, and does not cause any increase in the killing effect of MMS.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940000136