Consumption of ellagic acid and dihydromyricetin synergistically protects against UV-B induced photoaging, possibly by activating both TGF-β1 and wnt signaling pathways

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 178; pp. 92 - 100
• Main Authors: Moon, Na Rang, Kang, Suna, Park, Sunmin
• Format: Journal Article
• Language: English
• Published: Switzerland Elsevier B.V 01.01.2018
• Subjects: Inflammation; Oxidative stress; Photo-damage; TGF-β1 signaling; Wnt signaling; UV; NO; Oxidative stress; DHM; Px; SOD; Inflammation; IL-1β; MED; DKK1; NF-κB; Wnt signaling; TNF-α; TGF-β1 signaling; MMP-1; ROS; TGF-β; iNOS; Photo-damage; GSH; EGA; PCR
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2017.11.004

Ellagic acid (EGA) and dihydromyricetin (DHM) are both found in fruits and vegetables are used for anti-aging treatment for the skin. The anti-photoaging efficacy of EGA and DHM was investigated in UV-B irradiated skin in vivo and the involvement of transforming growth factor (TGF)-β1 and wnt signaling pathways were examined in vitro. HaCaT cells were treated with either 50μM EGA, 50μM DHM or 25μM EGA+25μM DHM before 100mJ/cm2 UV-B exposure, and then oxidative stress and inflammation was measured. The involvement of TGF-β1 and wnt signaling was measured using their inhibitors, respectively, in HaCaT cells. Mice were fed a high fat diet with either 0.7% cellulose, 0.7% EGA, 0.7% DHM or 0.35% EGA+0.35% DHM for 3weeks and the dorsal skin of the mice had UV-B irradiation. 3% cellulose, 3% EGA, 3% DHM or 1.5% EGA+1.5% DHM in 1,3-buthylene glycol was applied onto the dorsal skin at 30min before 1 MED UV-B exposure. In 100mJ/cm2 UVB irradiation, EGA and DHM mainly decreased oxidative stress and inflammation, respectively in HaCaT cells. Their activities were blocked by the TGF-β1 inhibitor, indicating their actions were mediated by TGF-β1 signaling (TGF-β1➔pSmad3➔Smad7). DHM enhanced wnt signaling by increasing β-catenin and decreasing Dickkopf-related protein-1. In mice, 1 MED UV-B exposure induced sunburn, redness, and blistering. EGA, DHM and especially EGA+DHM lessened their severity. UV-B increased epidermal thickness and damaged epidermal nucleus and cell structures. DHM and especially EGA+DHM prevented damage to the nucleus and cell structures. Expressions of circulating and dorsal skin IL-1β and TNF-α mRNA were lower in descending order of: control, EGA, DHM, EGA+DHM and normal-control. In conclusion, the consumption of EGA+DHM had a synergistically protective action against UV-B damage in the skin tissues of mice and HaCaT cells, and it may be associated with activating of both TGF-β1 and wnt signaling. [Display omitted] •Ellagic acid (EGA) and dihydromyricetin (DHM) decreased oxidative stress.•EGA and DHM reduced inflammation by activating TGF-β1 signaling in HaCaT cells.•DHM enhanced wnt signaling by increasing β-catenin and decreasing DKK1.•EGA+DHM synergistically lessened the severity of photo-aging in mice.•EGA+DHM synergistically prevented the increase of epidermal thickness and damage.