Random access three-dimensional two-photon microscopy

We propose a two-photon microscope scheme capable of real-time, three-dimensional investigation of the electric activity pattern of neural networks or signal summation rules of individual neurons in a 0.6 mm x 0.6 mm x 0.2 mm volume of the sample. The points of measurement are chosen according to a...

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Bibliographic Details
Published inApplied optics (2004) Vol. 46; no. 10; p. 1860
Main Authors Rózsa, Balázs, Katona, Gergely, Vizi, E Sylvester, Várallyay, Zoltán, Sághy, Attila, Valenta, Lásló, Maák, Pál, Fekete, Júlia, Bányász, Akos, Szipocs, Róbert
Format Journal Article
LanguageEnglish
Published United States 01.04.2007
Subjects
Action Potentials - physiology
Cells, Cultured
Fiber Optic Technology - instrumentation
Humans
Image Interpretation, Computer-Assisted - methods
Imaging, Three-Dimensional - instrumentation
Imaging, Three-Dimensional - methods
Microscopy, Fluorescence, Multiphoton - instrumentation
Microscopy, Fluorescence, Multiphoton - methods
Nerve Net - physiology
Neurons - cytology
Neurons - physiology
Optical Fibers
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Summary:We propose a two-photon microscope scheme capable of real-time, three-dimensional investigation of the electric activity pattern of neural networks or signal summation rules of individual neurons in a 0.6 mm x 0.6 mm x 0.2 mm volume of the sample. The points of measurement are chosen according to a conventional scanning two-photon image, and they are addressed by separately adjustable optical fibers. This allows scanning at kilohertz repetition rates of as many as 100 data points. Submicrometer spatial resolution is maintained during the measurement similarly to conventional two-photon microscopy.
ISSN:1559-128X
DOI:10.1364/AO.46.001860