Steroidogenesis in porcine follicle cells cultured in serum-free media

The purpose of this study was to determine the steroid production of cultured porcine follicle cells grown in serum-free media. Theca cells (TC) and granulosa cells (GC) from large porcine follicles (greater than 8 mm) were dispersed and plated as monolayer cultures in serum-free media. Media were r...

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Bibliographic Details
Published inGynecologic and obstetric investigation Vol. 29; no. 4; p. 285
Main Authors Stein, A L, diZerega, G S, Tonetta, S A
Format Journal Article
LanguageEnglish
Published Switzerland 01.01.1990
Subjects
Animals
Culture Media
Female
Gonadal Steroid Hormones - biosynthesis
Granulosa Cells - metabolism
Growth Substances - pharmacology
Swine
Theca Cells - metabolism
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Summary:The purpose of this study was to determine the steroid production of cultured porcine follicle cells grown in serum-free media. Theca cells (TC) and granulosa cells (GC) from large porcine follicles (greater than 8 mm) were dispersed and plated as monolayer cultures in serum-free media. Media were removed from the cultures at 3, 6, 12, 24, 48, 72, 96, and 120 h and assayed for estradiol, progesterone, testosterone and androstenedione. Both GC and TC were capable of producing progesterone, androgens and estradiol in serum-free media. GC were responsive to human chorionic gonadotropin and follicle-stimulating hormone while TC were only responsive to human chorionic gonadotropin. Growth factors, particularly insulin, appeared to enhance ovarian cells' steroidogenesis.
ISSN:0378-7346
1423-002X
DOI:10.1159/000293336