Isolation and characterization of a type I gene encoding a light-harvesting chlorophyll a/b-binding protein of photosystem II in peach

• Published in: Journal of the American Society for Horticultural Science Vol. 123; no. 4; pp. 493 - 499
• Main Authors: Chung, K.H, Buetow, D.E, Korban, S.S
• Format: Journal Article
• Language: English
• Published: 01.07.1998
• Subjects: AMINO ACID SEQUENCES; ARN MENSAJERO; ARN MESSAGER; BINDING PROTEINS; CHEMICAL COMPOSITION; CHLOROPHYLLE; CHLOROPHYLLS; CLOROFILAS; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; CULTIVO DE TEJIDOS; CULTURE DE TISSU; DARKNESS; EXPRESION GENICA; EXPRESSION DES GENES; FEUILLE; FOTOSISTEMAS; GENBANK/36064; GENE; GENE EXPRESSION; GENES; HOJAS; LEAVES; LIGHT; LUMIERE; LUZ; MESSENGER RNA; MOLECULAR SEQUENCE DATA; NUCLEOTIDE SEQUENCE; OBSCURIDAD; OBSCURITE; PHOTOSYSTEME; PHOTOSYSTEMS; PROTEINAS AGLUTINANTES; PROTEINE DE LIAISON; PRUNUS PERSICA; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; SIGNAL PEPTIDE; STEMS; TALLO; TIGE; TISSUE CULTURE
• ISSN: 0003-1062; 2327-9788
• DOI: 10.21273/JASHS.123.4.493

A nuclear gene, Lhcb1*Pp1, encoding a light-harvesting chlorophyll a/b-binding protein of photosystem II has been isolated from peach [Prunus persica (L.) Batsch. 'Stark Earliglo'] leaf genomic DNA, cloned, and sequenced. This gene encodes a precursor polypeptide of 267 amino acids with a transit peptide of 34 and a type I mature protein of 233 amino acids. The amino acid sequence of the mature polypeptide is 89% to 94% and 80% to 94% similar to those encoded by type I Lhcb genes of annual and other woody plants, respectively. In contrast, the amino acid sequence of the peach transit peptide is less conserved being 47% to 69% similar to those of annual plants and only 17% to 22% similar to those of other woody plants. The peach gene was used as a probe for Lhcb gene expression. Lhcb mRNA is detected in leaves of field-grown trees during June to October. Lhcb mRNA is detected at a high level in leaves of peach shoots grown in tissue culture in the light, but only at a trace level in leaves grown in the dark. Some Lhcb genes appear to be light-modulated in stems. Lhcb1*Pp1 contains four potential polyadenylation sites. S1 nuclease analysis detected transcripts of the sizes expected from each of the four polyadenylation sites. All four are found in leaves of light-grown shoots and of field-grown trees throughout the growing season. In contrast, only three are detected in stems of light-grown shoots