Interaction of mithramycin with isolated GC and CG sites

We have studied the interaction of the GC-specific, minor groove-binding ligand, mithramycin, with cloned DNA inserts containing isolated GC and CG sites flanked by regions of (AT)n and An.Tn using DNase I and hydroxyl radical footprinting. We find that mithramycin binds to GC better than CG and tha...

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Bibliographic Details
Published inJournal of molecular recognition Vol. 7; no. 3; p. 189
Main Authors Carpenter, M L, Cassidy, S A, Fox, K R
Format Journal Article
LanguageEnglish
Published England 01.09.1994
Subjects
Adenine - chemistry
Base Sequence
Binding Sites
Carbohydrate Sequence
Cytosine - chemistry
Deoxyribonuclease I
DNA - chemistry
Electrophoresis, Polyacrylamide Gel
Guanine - chemistry
Hydroxyl Radical
Molecular Sequence Data
Molecular Structure
Plicamycin - chemistry
Thymine - chemistry
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Summary:We have studied the interaction of the GC-specific, minor groove-binding ligand, mithramycin, with cloned DNA inserts containing isolated GC and CG sites flanked by regions of (AT)n and An.Tn using DNase I and hydroxyl radical footprinting. We find that mithramycin binds to GC better than CG and that AGCT is a better site than TGCA. Sites flanked by (AT)n appear to be bound better than those surrounded by An.Tn. Although no footprints are produced at T9GCA9 and T15CGA15, DNase I cleavage is enhanced within the GC sites suggesting that there is some interaction with the ligand. Mithramycin also alters the DNase I cleavage of (GA)n.(CT)n.
ISSN:0952-3499
DOI:10.1002/jmr.300070306