Electrochemical Determination of Levofloxacin Using Poly(Pyrogallol Red) Modified Glassy Carbon Electrode

• Published in: ChemistrySelect (Weinheim) Vol. 7; no. 41
• Main Authors: Koçak, Çağrı Ceylan, Aslışen, Burak, Karabiberoğlu, Şükriye, Özdokur, Kemal Volkan, Aslan, Avni, Koçak, Süleyman
• Format: Journal Article
• Language: English
• Published: 07.11.2022
• Subjects: Amperometry; Levofloxacin; Pharmaceutic; Poly(Pyrogallol Red); Voltammetry
• ISSN: 2365-6549; 2365-6549
• DOI: 10.1002/slct.202201864

In this paper, an electrochemical sensor for levofloxacin detection was developed by electrochemical polymerization of pyrogallol red (PGR) on the glassy carbon electrode (GCE) surfaces. Surface morphology and electrical properties of the Poly(PGR)/GCE obtained was characterized by SEM and EIS techniques. Voltammetric behaviour of the levofloxacin was found pH dependent, and the best response was obtained at pH 6.0 PBS. By monitoring the peak current at around 0.9 V, a wide linear range of calibration graph: from 0.2 μM‐ 15 μM LEV and 15 μM‐ 355 μM for levofloxacin, and very low detection limit of 97 nM were achieved with amperometry. Selectivity of the method developed was proven in the presence of possible interfering substances and the method was successfully employed for levofloxacin detection in pharmaceutical tablet and synthetic urine sample. The electrochemical determination of levofloxacin, one of the antibiotics used for the treatment of SARS‐CoV‐2 was developed. The detection limit of levofloxacin in the pyrogallol red(PGR) thin film modified electrode was found to be 97 nM in pH 6 PBS medium.