Control of bovine tick ['Rhipicephalus' ('Boophilus') 'microplus'] with 'Brunfelsia uniflora' leaf extract

• Published in: Australian Journal of Crop Science Vol. 13; no. 6; pp. 903 - 910
• Main Authors: Sugauara, Elaine Yae Yamashita, Sugauara, ElisângelaYumi, Sugauara, Rosangela Rumi, Bortolucci, Wanessa de Campos, Oliveira, Herika Line Marko de, Silva, Eloísa Schneider, Campos, Caio Franco de Araújo Almeida, Gonçalves, José Eduardo, Colauto, Nelson Barros, Gazim, Zilda Cristiani, Linde, Giani Andrea
• Format: Journal Article
• Language: English
• Published: Lismore, N.S.W Southern Cross Publishers 01.06.2019
• Subjects: Acaricides; Analysis; Antioxidants; Effect of chemicals on; Linolenic acids; Physiological effect; Plants; Rhipicephalus; Soils
• ISSN: 1835-2693; 1835-2707
• DOI: 10.21475/ajcs.19.13.06.p1653

Bovine tick has caused losses in livestock production profitability in Brazil. However, tick control has caused resistance of these ectoparasites against utilized acaricides. Alternative tick controls have been utilizing plants as sources of effective botanical acaricides. 'Brunfelsia uniflora' is a Brazilian plant with antimicrobial and antioxidant activity; however, there are no reports on its acaricidal activity. Therefore, this study aimed to evaluate the chemical composition of B. 'uniflora' leaf ethanolic extract and its efficiency to control bovine tick in 'vitro' and free-living stage 'ex situ'. The crude leaf extract was analyzed by gas chromatographer coupled to mass spectrometer (GC-MS) with identification of 17 compounds. The major compounds were phytol (22.96%), 9,12,15-octadecatrienoic acid, ethyl ester (Z,Z,Z) (21.18%), hexadecanoic acid, ethyl ester (12.74%) and vitamin E (8.77%). The crude extract presented acaricidal activity in 'vitro' against ingurgitated adult females, larvae and eggs of bovine tick. The LC99.9 for larvae was 103.21 mg mL-1 in 'in vitro' tests and was 100% efficient for 'ex situ' larva test (free-living stage). B. 'uniflora' leaf extract is an alternative for the control of the bovine tick cycle, mainly in the free-living stage (non-parasitic stage) under field conditions.