A “Clickable” Electrodeposited Polymer Films Based on 3‐Ethynylthiophene for the Covalent Immobilization of Proteins. Application to a Label‐free Electrochemical Immunosensor for Escherichia Coli and Staphylococcus Aureus Determination

An original electrodeposited polymer film, based on 3‐ethynylthiophene, for covalent immobilization of proteins via “click” reaction of copper catalyzed azide‐alkyne cycloaddition was developed. The best characteristics have demonstrated the layer‐by‐layer immobilized 3,4‐ethylendioxythiophene‐3‐eth...

Full description

Saved in:
Bibliographic Details
Published inElectroanalysis (New York, N.Y.) Vol. 33; no. 12; pp. 2469 - 2475
Main Authors Svalova, T. S., Medvedeva, M. V., Kozitsina, A. N.
Format Journal Article
LanguageEnglish
Published 01.12.2021
Subjects
3,4-ethylendioxythiophene
3-ethynylthiophene
copper catalysed azide-alkyne cycloaddition
covalent immobilization of proteins
Electrochemical polymerization
label-free electrochemical immunosensor
layer-by-layer electrodeposition
Online AccessGet full text

Cover

More Information
Summary:An original electrodeposited polymer film, based on 3‐ethynylthiophene, for covalent immobilization of proteins via “click” reaction of copper catalyzed azide‐alkyne cycloaddition was developed. The best characteristics have demonstrated the layer‐by‐layer immobilized 3,4‐ethylendioxythiophene‐3‐ethynylthiophene composite film. The bovine serum albumin and antibodies have been successfully immobilized on the surface of planar platinum electrode. The approach has been applied to label‐free electrochemical immunosensors for Escherichia coli and Staphylococcus aureus determination. The immunosensors demonstrated LODs 7.2 CFU/mL for Escherichia coli and 15.9 CFU/mL for Staphylococcus aureus, linear range 102–106 CFU/mL and could be promising for environmental and food control.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.202100320