Endophytic species of Colletotrichum associated with mango in northeastern Brazil
Endophytic species of Colletotrichum associated with Mangifera indica (mango) are poorly understood. In this study, Colletotrichum species were isolated from mango in Pernambuco State, Brazil. There were significant differences in isolation frequencies of Colletotrichum species among sites and plant...
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Published in | Fungal diversity Vol. 67; no. 1; pp. 181 - 202 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.07.2014
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Subjects | |
Online Access | Get full text |
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Summary: | Endophytic species of
Colletotrichum
associated with
Mangifera indica
(mango) are poorly understood. In this study,
Colletotrichum
species were isolated from mango in Pernambuco State, Brazil. There were significant differences in isolation frequencies of
Colletotrichum
species among sites and plant tissues. Mature leaf blades were colonized by most
Colletotrichum
isolates at the majority of sites. Partial sequences of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of 97
Colletotrichum
isolates were amplified as an initial measure of genetic diversity. Phylogenetic analysis with a subset of 22 isolates were performed based on a multi-locus dataset (ACT, TUB2, CAL, CHS-1, GAPDH, ITS) followed by Apn2/MAT IGS sequence-analysis for isolates within the
C. gloeosporioides
species complex. Molecular analysis associated with phenotypic characteristics revealed six previously described species [
C. asianum
,
C. cliviae
,
C. dianesei
(syn.
C. melanocaulon
),
C. fructicola
,
C. karstii
and
C. tropicale
] and one new species. This new species is introduced as
C. endomangiferae
. All species isolated were pathogenic on mango fruits but varied in their virulence. There was no distribution pattern of species among sites and plant tissues, although
C. asianum
was the most prevalent species at all sites and in all plant tissues studied. Five previously reported
Colletotrichum
species causing anthracnose in mango fruits in northeastern Brazil were also recovered as endophytes. |
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ISSN: | 1560-2745 1878-9129 |
DOI: | 10.1007/s13225-014-0293-6 |