Hepatocyte growth factor upregulates thymosin β4 in human umbilical vein endothelial cells

Hepatocyte growth factor (HGF) is a mesenchymal-derived cytokine. It exerts in vitro a motogenic effect on various target cells, which is displayed either by cell scattering, locomotion, and migration during the wound repair process of cultured cells, or invasiveness through the extracellular matrix...

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Published inBiochemical and biophysical research communications Vol. 296; no. 2; pp. 401 - 405
Main Authors Oh, In Suk, So, Sang Sup, Jahng, Kwang Yeop, Kim, Hwan Gyu
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.08.2002
Subjects
DD-PCR
HGF
HUVECs
MMP-2
Thymosin β4
MMP-2
Thymosin β4
HGF
HUVECs
DD-PCR
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Summary:Hepatocyte growth factor (HGF) is a mesenchymal-derived cytokine. It exerts in vitro a motogenic effect on various target cells, which is displayed either by cell scattering, locomotion, and migration during the wound repair process of cultured cells, or invasiveness through the extracellular matrix. Although it is known that HGF influences the motogenic effect of endothelial cells, the precise effects of HGF during angiogenesis are still poorly understood. To identify genes regulated via HGF signaling in HUVECs, we used the differential display polymerase chain reaction. In this study, thymosin β4 was found to be differentially expressed in HGF-treated HUVECs compared with control. Data from HPLC profile and induction of MMPs indicate that HGF may affect the biological behavior of HUVECs through a combination of the direct effects of HGF itself and indirect effects mediated via induction of thymosin β4 in vitro.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)00888-4