Establishment and Validation of a Time-Resolved Fluoroimmunoassay for Chlorpromazine

• Published in: Food analytical methods Vol. 5; no. 4; pp. 625 - 630
• Main Authors: Huang, Biao, Zhao, Lili, Sun, Junming, Zhang, Jue, Zhang, Yi, Zhang, Kai, Jin, Jian, Xie, Minhao
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.08.2012
• Subjects: Analytical Chemistry; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Food Science; Microbiology; Time-resolved fluoroimmunoassay; Chlorpromazine; Monoclonal antibody
• ISSN: 1936-9751; 1936-976X
• DOI: 10.1007/s12161-011-9286-1

The objective of this study is to establish a highly sensitive, time-resolved fluoroimmunoassay (TRFIA) for the rapid detection of chlorpromazine (CPZ). The antigen CPZ-OVA was used to coat a microtiter plate to compete with free chlorpromazine for binding to the anti-chlorpromazine monoclonal antibody. Europium (Eu 3+ )-labeled goat anti-mouse antibody was used as the tracer. The labeling rate of Eu 3+ -labeled goat anti-mouse antibody was 10.5 Eu 3+ /antibody. The average intra- and inter-batch coefficients of variation were 4.0% and 6.4%, respectively, and the average recovery rate was 90.27%. The sensitivity was 0.2 μg/L over a measurable range of 0.2–50 μg/L. The ED 20 , ED 50 , and ED 80 were 1.17, 4.85, and 17.12 μg/L, respectively. CPZ-TRFIA is one of the most sensitive methods for CPZ detection. This analytical method demonstrates good stability and a wide measurable range, demonstrating its potential for practical applications.