Development of Recombinant Matrix Metalloproteinase-11 Based Sandwich ELISA for the Diagnosis of Canine Mammary Tumor

Certain matrix metalloproteinases like MMP-11 (Stromelysin-3) and MMP-7 (Matrilysin) are reported to be highly expressed in breast cancer, so they may act as marker enzymes for breast cancer progression. Hyperimmune sera were raised against heterologously expressed recombinant canine MMP-11 in rabbi...

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Published inProceedings of the National Academy of Sciences, India. Section B: Biological sciences Vol. 83; no. 2; pp. 181 - 185
Main Authors Sunil Kumar, B. V., Aswani Kumar, K., Padmanath, K., Saxena, Meeta, Sharma, Bhaskar, Kataria, Meena
Format Journal Article
LanguageEnglish
Published India Springer-Verlag 01.06.2013
Subjects
Behavioral Sciences
Biomedical and Life Sciences
Life Sciences
Nucleic Acid Chemistry
Plant Biochemistry
Research Article
Matrix metalloproteinase-11
Mammary tumor
Diagnosis
Sandwich ELISA
Canine
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Summary:Certain matrix metalloproteinases like MMP-11 (Stromelysin-3) and MMP-7 (Matrilysin) are reported to be highly expressed in breast cancer, so they may act as marker enzymes for breast cancer progression. Hyperimmune sera were raised against heterologously expressed recombinant canine MMP-11 in rabbits and guinea pigs to standardize sandwich ELISA (sELISA) for relative quantification of circulating MMP-11 for the diagnosis of canine mammary tumor ( n  = 50). The test was found to be 98 % sensitive and 92 % specific at receiver-operating characteristics (ROC) with respect to histopathological staining in diagnosing canine mammary tumors. The area under the ROC curve (AUC) of rMMP-11 based sELISA, which represented a summary statistics of the overall diagnostic performance of the test also indicated that it could be used as a suitable test for diagnosing mammary neoplasms.
ISSN:0369-8211
2250-1746
DOI:10.1007/s40011-012-0127-9