Development of Recombinant Matrix Metalloproteinase-11 Based Sandwich ELISA for the Diagnosis of Canine Mammary Tumor
Certain matrix metalloproteinases like MMP-11 (Stromelysin-3) and MMP-7 (Matrilysin) are reported to be highly expressed in breast cancer, so they may act as marker enzymes for breast cancer progression. Hyperimmune sera were raised against heterologously expressed recombinant canine MMP-11 in rabbi...
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Published in | Proceedings of the National Academy of Sciences, India. Section B: Biological sciences Vol. 83; no. 2; pp. 181 - 185 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
India
Springer-Verlag
01.06.2013
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Subjects | |
Online Access | Get full text |
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Summary: | Certain matrix metalloproteinases like MMP-11 (Stromelysin-3) and MMP-7 (Matrilysin) are reported to be highly expressed in breast cancer, so they may act as marker enzymes for breast cancer progression. Hyperimmune sera were raised against heterologously expressed recombinant canine MMP-11 in rabbits and guinea pigs to standardize sandwich ELISA (sELISA) for relative quantification of circulating MMP-11 for the diagnosis of canine mammary tumor (
n
= 50). The test was found to be 98 % sensitive and 92 % specific at receiver-operating characteristics (ROC) with respect to histopathological staining in diagnosing canine mammary tumors. The area under the ROC curve (AUC) of rMMP-11 based sELISA, which represented a summary statistics of the overall diagnostic performance of the test also indicated that it could be used as a suitable test for diagnosing mammary neoplasms. |
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ISSN: | 0369-8211 2250-1746 |
DOI: | 10.1007/s40011-012-0127-9 |