Strategies for uncovering stable isotope tracing patterns between cell populations

• Published in: Current opinion in biotechnology Vol. 83; p. 102991
• Main Authors: MacPherson, Sarah, Duncan, Kyle D, Goodlett, David R, Lum, Julian J
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2023
• ISSN: 0958-1669; 1879-0429; 1879-0429
• DOI: 10.1016/j.copbio.2023.102991

Despite practical complexities, isotope tracing studies in humans are becoming increasingly feasible. However, several technological challenges need to be addressed in order to take full advantage of human tracing studies. First, absolute metabolic flux measurements in mice are not so easily applied to human models, given that tissue resection is restricted to a single surgical time point. Second, isotope tracing has yet to be employed to detect metabolic differences between cells types in vivo. Here, we discuss the current models and propose an alternative, liquid tumor environment, that could overcome these limitations. Furthermore, we highlight current strategies used to maintain isotopolog enrichment following cell isolation techniques to facilitate cell-type-specific analysis. [Display omitted] •Challenges of using isotope tracers within the human tumor microenvironment (TME).•Current models and analytics used for isotope tracing in humans.•Strategies for uncovering cell-specific isotope tracing within the human TME.