A Sensitive, Selective New Analytical Reagent, 2, 6-Diacetylpyridine bis-4-phenyl-3-thiosemicarbazone for Extractive Spectrophotometric Determination of Mo(VI) in Food Samples

• Published in: Food analytical methods Vol. 2; no. 2; pp. 141 - 148
• Main Authors: Reddy, S. Adinarayana, Reddy, K. Janardhan, Narayana, S. Lakshmi, Rao, Y. Subba, Ramachandraiah, C., Reddy, A. Varada
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.06.2009
• Subjects: Analytical Chemistry; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Food Science; Microbiology; 2, 6-Diacetylpyridine bis-4-phenyl-3-thiosemicarbazone (2, 6-DAPBPTSC); Food Samples; Molybdenum(VI)
• ISSN: 1936-9751; 1936-976X
• DOI: 10.1007/s12161-008-9050-3

Synthesized a new reagent 2, 6-Diacetylpyridine bis-4-phenyl-3-thiosemicarbazone (2, 6-DAPBPTSC) characterized and is proposed as a sensitive and selective analytical reagent for the extractive spectrophotometric determination of molybdenum(VI) at pH 3.5 to form a yellowish orange colored 1:1 chelate complex. The absorbance was measured at a maximum wavelength, 500 nm. This method obeys Beer’s law in the concentration range 0.90–9.00 μg mL −1 and the correlation coefficient of Mo(VI)–2,6-DAPBPTSC complex is 0.954, which indicates an adequate linearity between the two variables with good molar absorptivity and Sandell’s sensitivity, 1.212 × 10 4  L mol −1 cm −1 , 0.0079 μg cm −2 , respectively. The precision and accuracy of the method is checked with calculation of relative standard deviation ( n  = 5), 0.894% and the detection limit value is 0.0056 μg mL −1 . The instability constant of the method has been calculated by Asmus’ method as 6.476 × 10 –5 , at room temperature. The interfering effect of various cations and anions has also been studied. The method was successfully applied for the determination of Mo(VI) in food and water samples. The validity of the present method was evaluated in terms of Student ‘ t ’ test and Variance ‘ f ’ test, which indicates the significance of the present method an inter comparison of the experimental values, using atomic absorption spectrometer.