Antimalarial potential of extracts and isolated compounds from four species of genus Ammannia
The plants of genus Ammannia (Lythraceae) are being frequently used in traditional Chinese and Indian medicine to cure various diseases. The present study was designed to investigate the antimalarial potential of extracts and compounds from four species of genus Ammannia viz., A. multiflora , A. bac...
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Published in | Medicinal chemistry research Vol. 23; no. 2; pp. 870 - 876 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.02.2014
|
Subjects | |
Online Access | Get full text |
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Summary: | The plants of genus
Ammannia
(Lythraceae) are being frequently used in traditional Chinese and Indian medicine to cure various diseases. The present study was designed to investigate the antimalarial potential of extracts and compounds from four species of genus
Ammannia
viz.,
A. multiflora
,
A. baccifera
,
A. verticillata
, and
A. coccinea.
Properly dried and milled samples comprising of various plant parts were separately extracted with methanol and the extracts were fractionated with
n
-hexane, chloroform, and
n
-butanol. The chloroform (BR2),
n
-butanol (BR3) fractions of
A. baccifera
roots and methanol extract of
A. coccinea
(AC) showed potent in vitro antiplasmodial activity against
Plasmodium falciparum
NF-54 (IC
50
3.5, 2.7, and 4.5 μg/ml, respectively) with nontoxicity to Vero cells as evident from their high selectivity index (>57.41, >74.07, and >44.44, respectively). Apart from these, 11 extracts/fractions possessed significant (IC
50
14.5–40.2 μg/ml) antiplasmodial potential; most of which were nontoxic to Vero cells. The methanol extracts of
A. verticillata
aerial parts (VL) and AC,
n
-butanol fraction of
A. multiflora
(AM3) were evaluated for in vivo activity against rodent malaria parasite
P. berghei
, which showed percentage chemosuppression of parasitaemia in mice by 98.99, 63.33, and 60.00 %, respectively. The detailed phytochemical investigation of
A. multiflora
and
A. baccifera
afforded a total of 11 compounds; among them 4-hydroxy-α-tetralone, tetralone-4-
O
-β-
d
-glucopyranoside, and ammaniol exhibited moderate antiplasmodial activities (IC
50
31.5, 36.1, and 22.6 μg/ml, respectively). Due to high degree of selective antiplasmodial activity, these plants may find their use in antimalarial phytopharmaceuticals as well as in the development and discovery of safer and novel antimalarial leads. |
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ISSN: | 1054-2523 1554-8120 |
DOI: | 10.1007/s00044-013-0682-5 |