qPCR Identification of the kdr Allele F1534C in Voltage-Gated Sodium Channel Gene (vgsc) of the Major Mosquito Vectors Aedes aegypti and Aedes albopictus in Northern and Central Vietnam
Pyrethroid insecticides are currently the main tool for controlling major mosquito vectors of hemorrhagic fevers— Aedes aegypti and Ae . albopictus . The widespread use of insecticides has led to the spread of insecticide resistance mutations (or kdr mutations) in mosquito populations. A population...
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Published in | Russian journal of genetics Vol. 56; no. 4; pp. 460 - 469 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Moscow
Pleiades Publishing
01.04.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Pyrethroid insecticides are currently the main tool for controlling major mosquito vectors of hemorrhagic fevers—
Aedes aegypti
and
Ae
.
albopictus
. The widespread use of insecticides has led to the spread of insecticide resistance mutations (or
kdr
mutations) in mosquito populations. A population genetic study of
kdr
mutations provides information on changes in the genetic structure of mosquito populations as a result of anthropogenic impact and may be useful for making epidemiological prediction for the prevalence of Dengue and Chikungunya fevers. Multiplex PCR is traditionally used to identify
kdr
mutations in combination with sequencing of PCR fragments. We have developed a more productive method for identifying
kdr
mutations based on the SNP polymorphism analysis in the
vgsc
gene and qPCR. We identified the
kdr
mutation F1534C in
Aedes aegypti
and
Ae. albopictus
from the Ha Tinh province of Vietnam. In
Ae. albopictus
populations from Vietnam, this mutation was identified for the first time. |
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ISSN: | 1022-7954 1608-3369 |
DOI: | 10.1134/S1022795420040158 |