A new electrophoretic technique for determining catecholamines and their metabolites under the conditions of micellar electrokinetic chromatography format

It is shown that reversed-flow micellar electrokinetic chromatography in buffer electrolyte solutions with pH < 2.5 can be used to determine catecholamines and their metabolites. The effect of ion-pair (sodium octane sulfonate) and complexing agent (18-crown-6 and 4,13-diaza-18-crown-6) concentra...

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Bibliographic Details
Published inJournal of analytical chemistry (New York, N.Y.) Vol. 64; no. 5; pp. 518 - 523
Main Authors Kartsova, L. A., Ganzha, O. V.
Format Journal Article
LanguageEnglish
Published Dordrecht SP MAIK Nauka/Interperiodica 01.05.2009
Subjects
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Biogenic Amine
Sample Zone
HCOOH
Migration Time
Macrocycle
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Summary:It is shown that reversed-flow micellar electrokinetic chromatography in buffer electrolyte solutions with pH < 2.5 can be used to determine catecholamines and their metabolites. The effect of ion-pair (sodium octane sulfonate) and complexing agent (18-crown-6 and 4,13-diaza-18-crown-6) concentrations on the efficiency and selectivity of the separation of biological amines is studied. Complex formation in the macrocycle-analyte system is quantitatively estimated. An on-line version of preconcentration (sweeping) in the presence of 4 mM of 18-crown-6 and 5 mM of cyclohexylamine introduced into the sample matrix is proposed (the preconcentration factor is 900–1500).
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934809050153