A new electrophoretic technique for determining catecholamines and their metabolites under the conditions of micellar electrokinetic chromatography format
It is shown that reversed-flow micellar electrokinetic chromatography in buffer electrolyte solutions with pH < 2.5 can be used to determine catecholamines and their metabolites. The effect of ion-pair (sodium octane sulfonate) and complexing agent (18-crown-6 and 4,13-diaza-18-crown-6) concentra...
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Published in | Journal of analytical chemistry (New York, N.Y.) Vol. 64; no. 5; pp. 518 - 523 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
SP MAIK Nauka/Interperiodica
01.05.2009
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Subjects | |
Online Access | Get full text |
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Summary: | It is shown that reversed-flow micellar electrokinetic chromatography in buffer electrolyte solutions with pH < 2.5 can be used to determine catecholamines and their metabolites. The effect of ion-pair (sodium octane sulfonate) and complexing agent (18-crown-6 and 4,13-diaza-18-crown-6) concentrations on the efficiency and selectivity of the separation of biological amines is studied. Complex formation in the macrocycle-analyte system is quantitatively estimated. An on-line version of preconcentration (sweeping) in the presence of 4 mM of 18-crown-6 and 5 mM of cyclohexylamine introduced into the sample matrix is proposed (the preconcentration factor is 900–1500). |
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ISSN: | 1061-9348 1608-3199 |
DOI: | 10.1134/S1061934809050153 |