Diversity of cultivable bacteria isolated from the water column and bottom sediments of the Kara Sea shelf

• Published in: Microbiology (New York) Vol. 81; no. 4; pp. 484 - 491
• Main Authors: Suslova, M. Yu, Lipko, I. A., Mamaeva, E. V., Parfenova, V. V.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01.07.2012
• Subjects: Biomedical and Life Sciences; Experimental Articles; Life Sciences; Medical Microbiology; Microbiology; enzymatic activity; 16S rRNA; microbial community; identification; Kara Sea
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261712040157

In this work, the results of microbiological and molecular genetic investigation of the microorganisms inhabiting the Kara Sea and the adjacent Yenisei and Gydanskii Bays are presented. The microorganisms isolated from the samples collected in the studied area belonged to 4 phyla and 11 genera. Bacteria of two phyla, Firmicutes and Actinobacteria , prevailed; representatives of the Gammaproteobacteria and Bacteroidetes were isolated as well. According to their phenotypic properties, the obtained pure cultures were classified with the genera Streptomyces, Rhodococcus, Micrococcus, Bacillus, Pseudomonas, Acinetobacter, Flavobacterium , and Marinococcus . Analysis of the obtained nucleotide sequences of the 16S rRNA genes confirmed that the isolates belonged to the genus Bacillus . One strain was reidentified as Brevibacillus laterosporus , and two strains were identified Aeromonas piscicola and Plantibacter sp. The results of the study of the enzymatic activity of the obtained pure psychrotolerant cultures suggest that the microbial community is actively involved in the destruction processes occurring in the studied area.