Evaluation of some commonly used semiquantitative methods for urinary glucose and ketone determinations

• Published in: Diabetes (New York, N.Y.) Vol. 23; no. 5; pp. 474 - 479
• Main Authors: James, R C, Chase, G R
• Format: Journal Article
• Language: English
• Published: United States 01.05.1974
• Subjects: Acetoacetates - analysis; Autoanalysis; Diabetes Mellitus - diagnosis; Evaluation Studies as Topic; Glycosuria - diagnosis; Humans; Indicators and Reagents; Ketone Bodies - urine; Proteinuria - diagnosis; Reagent Strips; Statistics as Topic
• ISSN: 0012-1797; 1939-327X
• DOI: 10.2337/diab.23.5.474

The 2 Drop Clinitest, 5 Drop Clinitest, Tes-Tape, and Ketodiastix semiquantitative urinary glucose methods were evaluated using 300 urine samples from diabetic patients and comparing the results with those obtained by an AutoAnalyzer-glucose oxidase method. At high levels of glycosuria (above 1,500 mg.'100 ml.) the 2 Drop Clinitest method gave better quantitation than the other methods. In this range the Keto-diastix method often gave falsely low results. At intermediate levels of glycosuria (376 to 1,500 mg.'100 ml.) there appeared to be little or no difference among the methods. The 2 Drop Clinitest method was often insensitive to levels of urinary glucose below 376 mg.'100 ml. In this range the other three methods gave comparable results, with the exception that Tes-Tape was sometimes positive with normal levels (1 to 15 mg.'100 ml.) of glycosuria. Proteinuria and pregnancy had no effect on any of the methods. Acetest was a more sensitive and accurate indicator of urinary ketone levels than Keto-diastix.