DNA Binding and Cleavage Studies of Dichlorido[Bis(2-Ethyl-5-Methyl-1H-Imidazol-4-yl-κN3)Methane]Cobalt(II) Monohydrate

• Published in: Journal of chemical crystallography Vol. 42; no. 5; pp. 423 - 426
• Main Authors: Qian, Zhou, Qihe, Gao, Yanghui, Luo, Xiaomin, Qian, Jian, Xu, Baiwang, Sun
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.05.2012
• Subjects: Chemistry; Chemistry and Materials Science; Crystallography and Scattering Methods; Inorganic Chemistry; Organometallic Chemistry; Original Paper; Physical Chemistry; Cobalt(II) complex; Binding mode; Calf thymus DNA; Crystal structure
• ISSN: 1074-1542; 1572-8854
• DOI: 10.1007/s10870-011-0262-9

The synthesized complex [Co( L ) 2 Cl 2 ·H 2 O] ( L  = 5,5′-bis(2-ethyl-4-methylimidazol)methane) crystallizes in the monoclinic system, space group P 2(1)/m, with a  = 8.3927(7), b  = 12.1388(14), c  = 8.5860(9) Å, α  = 90.00, β  = 97.0450(10), γ  = 90.00°, Z  = 2, R 1  = 0.0420, wR 2  = 0.1018. The Co(II) lies on a mirror plane and displays a tetrahedral coordination with two N atoms of the imidazole ligand and two Cl atoms. The asymmetric unit also contains a solvate water molecule. The terminal C-atom of the ethyl group was disordered over two sites C6 and C6′ with equal site occupancy factors. The interaction of the complex with calf thymus DNA has been studied using emission spectroscopy, circular dichroism and gel electrophoresis measurements. The results indicate that the title complex binds to double-stranded DNA through partial intercalation and can cleave DNA. Graphical Abstract This article is about the interactions of a Cobalt(II) complex and DNA.