West Nile virus detection in urine

We report West Nile virus (WNV) RNA in urine collected from a patient with encephalitis 8 days after symptom onset. Viral RNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Sequence and phylogenetic analysis confirmed the PCR product to have > or = 99% similarity to the...

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Bibliographic Details
Published inEmerging infectious diseases Vol. 11; no. 8; p. 1294
Main Authors Tonry, Jessica H, Brown, Craig B, Cropp, Cecil B, Co, Juliene K G, Bennett, Shannon N, Nerurkar, Vivek R, Kuberski, Timothy, Gubler, Duane J
Format Journal Article
LanguageEnglish
Published United States 01.08.2005
Subjects
Aged
Antibodies, Viral - blood
Cluster Analysis
Humans
Male
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - chemistry
RNA, Viral - genetics
Sequence Alignment
Sequence Analysis, DNA
West Nile Fever - drug therapy
West Nile Fever - urine
West Nile virus
West Nile virus - genetics
West Nile virus - isolation & purification
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Summary:We report West Nile virus (WNV) RNA in urine collected from a patient with encephalitis 8 days after symptom onset. Viral RNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Sequence and phylogenetic analysis confirmed the PCR product to have > or = 99% similarity to the WNV strain NY 2000-crow3356.
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ISSN:1080-6040
1080-6059
DOI:10.3201/eid1108.0238