Measurement of Antibodies to Herpesvirus Types 1 and 2 in Human Sera

Abstract When varying dilutions of human sera were mixed with constant amounts of the two types of herpesvirus, it was observed that either type 1 virus was neutralized at a greater serum dilution than type 2, type 2 was neutralized at a greater serum dilution than type 1, or the two types were neut...

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Published inThe Journal of immunology (1950) Vol. 104; no. 3; pp. 599 - 606
Main Authors Rawls, William E, Iwamoto, Kumiko, Adam, Erwin, Melnick, Joseph L
Format Journal Article
LanguageEnglish
Published United States Am Assoc Immnol 01.03.1970
Subjects
Antibodies - analysis
Antigen-Antibody Reactions
Antigens
Culture Techniques
Female
Herpes Simplex - immunology
Humans
Immune Sera
Kidney - immunology
Neutralization Tests
Serotyping
Simplexvirus - immunology
Uterine Cervical Neoplasms - immunology
Virus Cultivation
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Summary:Abstract When varying dilutions of human sera were mixed with constant amounts of the two types of herpesvirus, it was observed that either type 1 virus was neutralized at a greater serum dilution than type 2, type 2 was neutralized at a greater serum dilution than type 1, or the two types were neutralized equally well. The relative antibody titer of type 2 virus to type 1 virus was found to correlate with the location of herpetic lesions experienced by the patients. When the ratio was expressed as an index (II/I index), sera from persons with oral herpesvirus type 1 infections, for the most part, yielded values of less than 85 whereas sera from persons with genital herpesvirus type 2 infections yielded values of 85 or greater. The same relationship was demonstrated using either the plaque-reduction or the microneutralization test. The plaque-reduction test, microneutralization test and neutralization kinetics test were compared by analyzing a group of sera for herpesvirus type 2 antibodies by all three methods. There was agreement between the three methods in 83% to 89% of the determinations. Thus antibodies to the two herpesviruses can be quantitated in the same serum by neutralization techniques employing varying serum dilutions and a constant amount of virus, and the same antibody activity is measured as in the neutralization kinetics test.
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ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.104.3.599