Photoreduction of 2,6-Dichlorophenolindophenol by Diphenylcarbazide: A Photosystem 2 Reaction Catalyzed by Tris-Washed Chloroplasts and Subchloroplast Fragments

The use of diphenylcarbazide as an electron donor coupled to the photoreduction of 2,6-dichlorophenolindophenol by tris-washed chloroplasts or subchloroplast fragments provides a simple and sensitive assay for photosystem 2 of chloroplasts. By varying the concentration of tris buffer at pH 8.0 durin...

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Published inPlant physiology (Bethesda) Vol. 44; no. 11; pp. 1645 - 1649
Main Authors Vernon, Leo P., Elwood R. Shaw
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Physiologists 01.11.1969
Subjects
Chloroplasts
Molecules
Oxidation
Oxygen
Phosphates
Photochemical reactions
Photooxidation
Plants
Spinach
Wavelengths
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Summary:The use of diphenylcarbazide as an electron donor coupled to the photoreduction of 2,6-dichlorophenolindophenol by tris-washed chloroplasts or subchloroplast fragments provides a simple and sensitive assay for photosystem 2 of chloroplasts. By varying the concentration of tris buffer at pH 8.0 during an incubation period it is shown that the destruction of oxygen evolution activity is accompanied by a corresponding emergence of an ability to photooxidize diphenylcarbazide, as evidenced by absorbance changes due to diphenylcarbazide at 300 nm. The temperature-sensitive oxidation of diphenylcarbazide is inhibited by DCMU and by high ionic strengths. This activity appears to measure the primary photochemical reaction of photosystem 2.
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Contribution No. 365 from the C. F. Kettering Research Laboratory. This investigation was supported in part by Research Grant GB-8434 from the National Science Foundation.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.44.11.1645