Inheritance of lethal gene ‘luteus-Pa’ from cacao progenies obtained by self and cross-fertilization

• Published in: Revista Facultad Nacional de Agronomía, Medellín Vol. 70; no. 2; pp. 8123 - 8128
• Main Authors: Meza S, Zurel N, García Carrión, Luis Fernando, García Rodríguez, Patricia Vanessa
• Format: Journal Article
• Language: English; Portuguese
• Published: Bogota Universidad Nacional de Colombia 01.05.2017; Facultad de Ciencias Agrarias - Universidad Nacional de Colombia; Universidad Nacional de Colombia, Sede Medellín
• Subjects: AGRONOMY; Cacao; Chi-square (X2) test; Chi-square test; Cross-fertilization; Fertilization; Genes; Genetic crosses; Heredity; Homology; Lethal factor; Mendelian segregation; Offspring; Photosynthesis; Plant reproduction; Plastics; Progeny; Sandy soils; Seedlings; Seeds; Self-cross pollination; Self-fertilization; Statistical tests; Substrates; Self-cross pollination; Auto-alopolinización; Segregación mendeliana; Prueba chi-cuadrado (X2); Mendelian segregation; Lethal factor; Factor letal; Chi-square (X2) test; Cacao
• ISSN: 0304-2847; 2248-7026; 2248-7026
• DOI: 10.15446/rfna.v70n2.64502

With the aim to study the inheritance of lethal gene ‘luteus-Pa’ in three derivated progenies from self and cross-fertilization of cacao (Theobroma cacao L) varieties, a study was carried out during January to September 2013. As genetic material C-25, S-5 y Pa-150 varieties were utilized and this one were self and cross pollinated and their progenies were divided in two replicates with 100 seeds each and sowed in plastic recipients containing a substrate 2:1(soil: sand). In order to contrast theobserved segregation with its expected homologous 3:1 (monogenic inheritance), the Chi-square (X2) test was utilized. The results shown that only one from self-fertilized progeny of C-25 variety exhibited the mendelian segregation 3:1 in both replicates, so that it lead to infer this one is carrier of lethal gene ‘luteus-Pa’ in heterozygote condition whereas that the progeny from Pa-150 x C-25 cross no found none lethal seedling and submitted to Chi-square (X2) test it could be to infer this one no segregate in the proportion 3:1 therefore, the Pa-150 variety is no carrier of this lethal gene. A better comprehension on photobiological origin, supported by molecular markers and the functional genomics of this mutant can help to identify and understand the factors involve in the photosynthetic mechanisms.