Proteomic analysis of differential protein expression in early process of pancreatic regeneration in pancreatectomized rats
Aim: A broad-range proteomic approach was applied to investigate the complexity of the mechanisms involved in pancreatic regeneration for identification of new targets of diabetes treatment and potential markers of pancreatic stem cells. Methods: A regeneration pancreatic model was induced by 90% pa...
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Published in | Acta pharmacologica Sinica Vol. 27; no. 5; pp. 568 - 578 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Nature Publishing Group
01.05.2006
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Subjects | |
Online Access | Get full text |
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Summary: | Aim: A broad-range proteomic approach was applied to investigate the complexity of the mechanisms involved in pancreatic regeneration for identification of new targets of diabetes treatment and potential markers of pancreatic stem cells. Methods: A regeneration pancreatic model was induced by 90% partial pancreatectomy (Px) in rats. Changes in the protein expression in regenerating rat pancreas on the third day after Px, as compared with rats that received sham surgery, were analyzed by using 2-D gel electrophoresis (2-DE), mass spectrometry (MS), and mass fingerprinting. Results: 2-DE revealed 91 spots with at least 1.5-fold in- creases in expression at 3 d after pancreatectomy and 53 differentially expressed proteins that were identified by peptide mass fingerprinting (PMF). These included cell growth-related, lipid and energy metabolism-related, protein and amino acid metabolism-related proteins, and signal transduction proteins. Vimentin, CKS, L-plastin, hnRNP A2/B 1, and AGAT are associated with embryogenesis and cell differentiation, and may be new potential pancreatic stem cells markers. Conclusion: The proteome profiling technique provided a broad-based and effective approach for the rapid assimilation and identification of adaptive protein changes during pancreas regeneration induced by pancreatectomy. Our data clarify the global proteome during the pancreatic proliferation and differentiation processes, which is important for better understanding of pancreatic regeneration and for discovering of protein biomarkers for pancreatic stem cells. |
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Bibliography: | R657.5 stem cell partial pancreatectomy; regeneration; metabolism; stem cell; pancreas proteomics regeneration partial pancreatectomy metabolism pancreas proteomics 31-1347/R ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1671-4083 1745-7254 |
DOI: | 10.1111/j.1745-7254.2006.00317.x |