Analysis of viral diversity in dogs with acute gastroenteritis from Brazilian Amazon

• Published in: Infection, genetics and evolution Vol. 123; p. 105637
• Main Authors: de Deus, Danielle Rodrigues, Siqueira, Jones Anderson Monteiro, Maués, Marcelino Antonio Costa, de Fátima Mesquita de Figueiredo, Márcia Janete, Júnior, Edivaldo Costa Sousa, da Silva Bandeira, Renato, da Costa Pinheiro, Kenny, Teixeira, Dielle Monteiro, da Silva, Luciana Damascena, de Fátima dos Santos Guerra, Sylvia, da Silva Soares, Luana, Gabbay, Yvone Benchimol
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2024
• Subjects: Animals; Brazil - epidemiology; Diarrhea; Dog Diseases - epidemiology; Dog Diseases - virology; Dogs; Feces - virology; Gastroenteritis - epidemiology; Gastroenteritis - veterinary; Gastroenteritis - virology; Genetic Variation; Genome, Viral; Metagenomics; Metagenomics - methods; Norovirus; Norovirus - classification; Norovirus - genetics; Norovirus - isolation & purification; Phylogeny; Virome; Viruses - classification; Viruses - genetics; Viruses - isolation & purification; Brazil; Diarrhea; Metagenomics; Norovirus; Virome; Dogs
• ISSN: 1567-1348; 1567-7257; 1567-7257
• DOI: 10.1016/j.meegid.2024.105637

Viral gastroenteritis is commonly reported in dogs and involves a great diversity of enteric viruses. In this research, viral diversity was investigated in dogs with diarrhea in Northern Brazil using shotgun metagenomics. Furthermore, the presence of norovirus (NoV) was investigated in 282 stool/rectal swabs of young/adult dogs with or without diarrhea from two public kennels, based on one-step reverse transcription polymerase chain reaction (RT-PCR) for genogroup VI and VII (GVI and GVII) and real-time RT-PCR for GI, GII, and GIV. Thirty-one viral families were identified, including bacteriophages. Phylogenetic analyses showed twelve complete or nearly complete genomes belonging to the species of Protoparvovirus carnivoran1, Mamastrovirus 5, Aichivirus A2, Alphacoronavirus 1, and Chipapillomavirus 1. This is the first description of the intestinal virome of dogs in Northern Brazil and the first detection of canine norovirus GVII in the country. These results are important for helping to understand the viral groups that circulate in the canine population. •Fecal samples from dogs with or without enteric signs were screened for norovirus.•First detected canine norovirus genogroup VII in fecal samples from Latin America.•The first description of the intestinal viroma of urban dogs in Northern Brazil.•Analysis of viral diversity had shown 31 viral families in cases of canine diarrhea.•The complete or nearly complete genome sequences were determined for twelve strains.