A cross-platform metabolomics workflow for volume-restricted tissue samples: application to an animal model for polycystic kidney disease

Metabolic profiling provides an unbiased view of the physiological status of an organism as a "function" of the metabolic composition of a measured sample. Here, we propose a simple LC-MS based workflow for metabolic profiling of volume-restricted samples, namely individual 20 μm-thick his...

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Published inMolecular bioSystems Vol. 13; no. 10; pp. 1940 - 1945
Main Authors Sánchez-López, E, Happé, H, Steenvoorden, E, Crego, A L, Marina, M L, Peters, D J M, Mayboroda, O A
Format Journal Article
LanguageEnglish
Published England 26.09.2017
Subjects
Animals
Chromatography, Liquid
Mass Spectrometry
Metabolomics - methods
Polycystic Kidney Diseases - metabolism
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Summary:Metabolic profiling provides an unbiased view of the physiological status of an organism as a "function" of the metabolic composition of a measured sample. Here, we propose a simple LC-MS based workflow for metabolic profiling of volume-restricted samples, namely individual 20 μm-thick histological sections of a mouse kidney. The main idea of this workflow is to re-use the material after an RPLC-MS run, namely using the volume remaining in the vial after injection, and then introducing a phase changing step to enable HILIC-MS analysis. To test the applicability of the workflow and its ability to extract valuable biological information, we applied it to an animal model of polycystic kidney disease (PKD).
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ISSN:1742-206X
1742-2051
DOI:10.1039/c7mb00245a