Isolation and identification of killer yeasts from Agave sap (aguamiel) and pulque

• Published in: World journal of microbiology & biotechnology Vol. 17; no. 6; pp. 557 - 560
• Main Authors: ESTRADA-GODINA, A. R, CRUZ-GUERRERO, A. E, LAPPE, P, ULLOA, M, GARCIA-GARIBAY, M, GOMEZ-RUIZ, L
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.08.2001; Springer Nature B.V
• Subjects: Agave; alcohols; Biological and medical sciences; Candida lusitaneae; Candida valida; Ethanol; Fermentation; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; killer cells; Kluyveromyces marxianus; Microbiology; Saccharomyces cerevisiae; Wines; Yeast; Yeasts; Central America; Mexico; America; Monocotyledones; Yeast; Fermentation starter; Agave; Fungi; Resistance; Alcoholic beverage; Alcoholic fermentation; Angiospermae; Isolate; Agavaceae; Ascomycetes; Spermatophyta; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1023/A:1012210106203

Wild killer yeasts have been identified as inhibitory to strains used as starters in the production of alcoholic beverages such as beer and wine; therefore, killer or killer-resistant strains have been sought for use in alcoholic fermentations. In the current paper a total of 16 strains belonging to six species were isolated. From two samples of Agave sap (aguamiel) the following yeast strains were isolated: Candida lusitaneae (1), Kluyveromyces marxianus var. bulgaricus (2), and Saccharomyces cerevisiae (capensis) (1). Additionally, in seven samples of pulque (the fermented product), the species C. valida (six strains), S. cerevisiae (chevalieri) (4), S. cerevisiae (capensis) (1), and K. marxianus var. lactis (1) were found. The killer strains were C. valida and K. marxianus var. lactis from pulque and K. marxianus var. bulgaricus from aguamiel. One strain of S. cerevisiae (chevalieri) isolated from pulque which did not show killer activity was, on the other hand, resistant to other killer strains and it had a remarkable ethanol tolerance, suggesting that this strain could be used for alcohol production.[PUBLICATION ABSTRACT]