An improved method for purification of lymphocytes

A method that combines glass-bead column filtration, Ficoll-Hypaque gradient separation, discontinuous sucrose gradient, and drastic reduction of cell transfers is described. The procedure gives a high yield of pure human lymphocytes from small amounts of blood, good preservation of B cell/T cell ra...

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Bibliographic Details
Published inAmerican journal of clinical pathology Vol. 68; no. 6; pp. 763 - 765
Main Authors Carpentieri, U, Thorpe, L, Sordahl, L A
Format Journal Article
LanguageEnglish
Published England 01.12.1977
Subjects
Adolescent
Cell Separation - methods
Centrifugation
Child
Filtration
Glass
Humans
Lymphocytes
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Summary:A method that combines glass-bead column filtration, Ficoll-Hypaque gradient separation, discontinuous sucrose gradient, and drastic reduction of cell transfers is described. The procedure gives a high yield of pure human lymphocytes from small amounts of blood, good preservation of B cell/T cell ratio, and sufficient material for subsequent biochemical studies.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0002-9173
1943-7722
DOI:10.1093/ajcp/68.6.763