Production of cholesterol oxidase by a newly isolated Rhodococcus sp

Fifteen strains of microorganisms with ability to degrade cholesterol were isolated. Among them a Gram-positive, non-motile, non-sporing bacterium with meso-DAP in the cell wall and with a rod-coccus cycle showed the highest ability for cholesterol degradation. It was identified as Rhodococcus sp. s...

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Published inWorld journal of microbiology & biotechnology Vol. 17; no. 7; pp. 731 - 737
Main Authors TABATABAEI YAZDI, M, MALEKZADEH, F, ZARRINI, Gh, FARAMARZI, M. A, KAMRANPOUR, N, KHALEGHPARAST, Sh
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.10.2001
Springer Nature B.V
Subjects
Bacteria
Biological and medical sciences
Biotechnology
Carbon sources
Cholesterol
Enzyme engineering
Enzymes
Fermentation
Fundamental and applied biological sciences. Psychology
Metabolites
Methods. Procedures. Technologies
Microbiology
Microorganisms
Production of selected enzymes
Rhodococcus
Yeasts
Culture medium
Enzyme
Production
Bacteria
Rhodococcus
Actinomycetes
Cholesterol oxidase
Oxidoreductases
Optimization
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Summary:Fifteen strains of microorganisms with ability to degrade cholesterol were isolated. Among them a Gram-positive, non-motile, non-sporing bacterium with meso-DAP in the cell wall and with a rod-coccus cycle showed the highest ability for cholesterol degradation. It was identified as Rhodococcus sp. strain 2C and was deposited by code 1633 in Persian type culture collection (PTCC). This strain was able to produce high levels of both extracellular and cell-bound cholesterol oxidases in media containing cholesterol as a sole carbon source. The effects of medium composition and physical parameters on cholesterol oxidase production were studied. The optimized medium was found to contain cholesterol 0.15% (w/v), yeast extract 0.3% (w/v), diammonium hydrogen phosphate 0.1% (w/v), Tween 80 (0.05%). The optimum pH and temperature for cholesterol oxidase production in optimized medium were found to be 8-30 °C respectively. Triton X-100 showed the greatest effect in releasing the cell-bound enzyme. The first and most probably the main metabolite of cholesterol degradation was purified and identified as 4-cholestene-3-one.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0959-3993
1573-0972
DOI:10.1023/A:1012993532686