An enzyme-free DNA walker that moves on the surface of functionalized magnetic microparticles and its biosensing analysis

• Published in: Chemical communications (Cambridge, England) Vol. 53; no. 60; pp. 8486 - 8488
• Main Authors: Li, Ningxing, Zheng, Jiao, Li, Chunrong, Wang, Xinxin, Ji, Xinghu, He, Zhike
• Format: Journal Article
• Language: English
• Published: England 25.07.2017
• Subjects: Bacteriophage T4 - enzymology; Biosensing Techniques; DNA - analysis; DNA, Catalytic - chemistry; DNA, Catalytic - metabolism; Magnetite Nanoparticles - chemistry; Polynucleotide 5'-Hydroxyl-Kinase - metabolism; Surface Properties

An enzyme-free stochastic DNA walker propelled by a single catalytic or double catalytic DNA assembly has been constructed. The application of the proposed DNA walking biosensor was successfully expanded to the detection of DNA and the enzymatic activity of T4 polynucleotide kinase.