Blue 'turn-on' fluorescent probes for the direct detection of free radicals and nitric oxide in Pseudomonas aeruginosa biofilms

Two novel cell-permeable and bacteria-compatible 'turn-on' fluorescent probes, designed to be compatible in a multi-dye system and to fluoresce in the blue region exhibiting emission maxima of 440-490 nm, were synthesized. The profluorescent nitroxide probe (11) was developed to visualize...

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Published inOrganic & biomolecular chemistry Vol. 14; no. 7; pp. 2272 - 2281
Main Authors Olia, Mina Barzegar Amiri, Zavras, Athanasios, Schiesser, Carl H., Alexander, Stefanie-Ann
Format Journal Article
LanguageEnglish
Published CAMBRIDGE Royal Soc Chemistry 21.02.2016
Subjects
Biofilms - growth & development
Cell Membrane Permeability
Chemistry
Chemistry, Organic
Copper - chemistry
Coumarins - chemistry
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - classification
Fluorescent Dyes - pharmacokinetics
Free Radicals - analysis
Microscopy, Confocal
Nitric Oxide - chemistry
Oxidation-Reduction
Physical Sciences
Pseudomonas aeruginosa
Science & Technology
Staining and Labeling
DISPERSAL
CYCLOADDITION
AZIDES
NITROXIDES
COMPLEXES
TERMINAL ALKYNES
SYSTEMS
BACTERIAL BIOFILMS
LIVING CELLS
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Summary:Two novel cell-permeable and bacteria-compatible 'turn-on' fluorescent probes, designed to be compatible in a multi-dye system and to fluoresce in the blue region exhibiting emission maxima of 440-490 nm, were synthesized. The profluorescent nitroxide probe (11) was developed to visualize and quantify free radical and redox processes, and the Cu(II)-complexed coumarin-based probe (16a) was developed for NO detection. Confocal laser-scanning microscopy and subsequent digital analysis of Pseudomonas aeruginosa biofilms stained with 11 or 16a determined that free radical and redox processes and NO generation occur predominantly in live cells during normal biofilm growth.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1477-0520
1477-0539
DOI:10.1039/c5ob02441b